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Review
. 2001 Feb;67(2):491-4.
doi: 10.1128/AEM.67.2.491-494.2001.

Electrophoretic mobility distributions of single-strain microbial populations

H C van der Mei  1 , H J Busscher
Affiliations
Review

Electrophoretic mobility distributions of single-strain microbial populations

H C van der Mei et al. Appl Environ Microbiol. 2001 Feb.
No abstract available

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Figures

FIG. 1
FIG. 1
Electrophoretic mobility distributions of E. coli Hu734 organisms suspended in 10 mM potassium phosphate (pH 6.3). The three distributions (μ1, μ2, and μ3) were measured on separately cultured bacterial strains, and each has its own SDpopulation. Typically, the μmean of μ1, μ2, and μ3 is presented in the literature as an electrophoretic mobility of −2.1 (±0.7) × 10−8 m2 V−1 s−1, with a standard deviation, SDcultures, from the three measurements.
FIG. 2
FIG. 2
Bimodal electrophoretic mobility distribution of Pseudomonas aeruginosa #3, suspended in phosphate-buffered saline (pH 7.0).
FIG. 3
FIG. 3
Electron micrograph of ruthenium red-uranyl acetate-stained (12) L. acidophilus RC14 cells after being subcultured 20 times. Individual organisms with a thick stainable layer as their outermost cell surface can be observed next to organisms devoid of a stainable layer (micrograph reprinted from the Journal of Microbiological Methods [9] with permission from Elsevier Science). The bar denotes 0.15 μm.
FIG. 4
FIG. 4
Optical densities as a function of vortexing time for S. salivarius HB (●) and S. oralis J22 (▴) in MATH analysis. Hexadecane was used as the hydrocarbon phase, while bacteria were suspended in 10 mM potassium phosphate, pH 5.0. Note that for S. salivarius HB, the optical density is reduced essentially to zero but that for S. oralis J22, a sizeable optical density remains, also after prolonged vortexing.
See this image and copyright information in PMC

References

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    1. Cowan M M, van der Mei H C, Stokroos I, Busscher H J. Heterogeneity of surfaces of subgingival bacteria as detected by zeta potential measurements. J Dent Res. 1992;71:1803–1806. - PubMed

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