Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
Comparative Study
. 2001 Feb;39(2):519-24.
doi: 10.1128/JCM.39.2.519-524.2001.

Multicenter comparison trial of DNA extraction methods and PCR assays for detection of Chlamydia pneumoniae in endarterectomy specimens

P Apfalter  1 , F Blasi, J Boman, C A Gaydos, M Kundi, M Maass, A Makristathis, A Meijer, R Nadrchal, K Persson, M L Rotter, C Y Tong, G Stanek, A M Hirschl
Affiliations
Comparative Study

Multicenter comparison trial of DNA extraction methods and PCR assays for detection of Chlamydia pneumoniae in endarterectomy specimens

P Apfalter et al. J Clin Microbiol. 2001 Feb.

Abstract

The reported rate of detection of Chlamydia pneumoniae DNA within atherosclerotic lesions by PCR varies between 0 and 100%. In this study, identical sets of coded experimental atheroma samples (n = 15) and spiked controls (n = 5) were analyzed by 16 test methods in nine centers by means of PCR. The positive controls were correctly identified to levels of 1, 0.1, and 0.01 inclusion bodies of C. pneumoniae/ml of tissue homogenate by 16 (100%), 11 (69%), and 3 (19%) of the test methods, respectively. Three out of 16 negative controls (19%) were rated positive. Positivity rates for atheroma samples varied between 0 and 60% for the different test methods, with the maximum concordant result for positivity being only 25% for one carotid artery sample. There was no consistent pattern of positive results among the various laboratories, and there was no correlation between the detection rates and the sensitivity of the assay used.

PubMed Disclaimer

References

    1. Blasi F, Denti F, Erba M, Cosentini R, Raccanelli R, Rinaldi A, Fagetti L, Esposito G, Roberti U, Allegra L. Detection of Chlamydia pneumoniae but not Helicobacter pylori in atherosclerotic plaques of aortic aneurysms. J Clin Microbiol. 1996;34:2677–2769. - PMC - PubMed
    1. Boman J, Gaydos C A, Quinn T C. Molecular diagnosis of Chlamydia pneumoniae infection. J Clin Microbiol. 1999;37:3791–3799. - PMC - PubMed
    1. Campbell L A, Perez Melgosa M, Hamilton D J, Kuo C C, Grayston J T. Detection of Chlamydia pneumoniae by polymerase chain reaction. J Clin Microbiol. 1992;30:434–439. - PMC - PubMed
    1. Fredricks D N, Relman D A. Application of polymerase chain reaction to the diagnosis of infectious diseases. Clin Infect Dis. 1999;29:475–488. - PubMed
    1. Gaydos C A, Fowler C L, Gill V J, Eiden J J, Quinn T C. Detection of Chlamydia pneumoniae by polymerase chain reaction-enzyme immunoassay in an immunocompromised population. Clin Infect Dis. 1993;17:718–723. - PubMed

MeSH terms

LinkOut - more resources