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Comparative Study
. 2001 Feb;39(2):758-61.
doi: 10.1128/JCM.39.2.758-761.2001.

Comparative evaluation of semiautomated COBAS AMPLICOR hepatitis B virus (HBV) monitor test and manual microwell plate-based AMPLICOR HBV MONITOR test

I J Marin  1 , M Poljak, K Seme, J Meglic-Volkar, M Maticic, G Lesnicar, V Brinovec
Affiliations
Comparative Study

Comparative evaluation of semiautomated COBAS AMPLICOR hepatitis B virus (HBV) monitor test and manual microwell plate-based AMPLICOR HBV MONITOR test

I J Marin et al. J Clin Microbiol. 2001 Feb.

Abstract

Comparative evaluation of the semiautomated COBAS AMPLICOR hepatitis B virus (HBV) MONITOR Test (COBAS-HBV) and manual AMPLICOR HBV MONITOR Test (AMPLICOR-HBV) on 208 serum samples revealed no significant difference in the sensitivities of the two assays. Twenty samples tested HBV DNA negative and 183 samples tested HBV DNA positive by both assays. Three samples tested positive by COBAS-HBV only and two samples tested positive by AMPLICOR-HBV only. HBV DNA concentrations determined by the two assays were significantly related (n = 183, r = 0.97, P < 0.0001), which indicates that COBAS-HBV could replace AMPLICOR-HBV. The major inconvenience of COBAS-HBV is the required performance of appropriate predilutions of high-titer samples in order to extend the narrow dynamic range of the assay.

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Figures

FIG. 1
FIG. 1
Correlation between COBAS-HBV- and AMPLICOR-HBV-determined HBV DNA concentrations in the analysis of HBV DNA in 183 serum samples.
See this image and copyright information in PMC

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