The technique of intracerebral microdialysis

Abstract

Intracerebral microdialysis is an increasingly popular experimental technique. A brief description of the principles of microdialysis is presented and the terms relevant to the procedure are defined. The methodology involved in conducting intracerebral microdialysis is described in detail. Factors influencing the outcome of analysis such as external stimuli, perfusion fluid, perfusion rate, temperature, probe placement, membrane characteristics, and timing of sample collection are discussed. The importance of maintaining the uniformity of the above-mentioned factors is stressed.