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. 2001 Jan 19;488(3):160-4.
doi: 10.1016/s0014-5793(00)02332-2.

Occurrence of ceramides and neutral glycolipids with unusual long-chain base composition in purified rat liver mitochondria

D Ardail  1 , I Popa, K Alcantara, A Pons, J P Zanetta, P Louisot, L Thomas, J Portoukalian
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Free article

Occurrence of ceramides and neutral glycolipids with unusual long-chain base composition in purified rat liver mitochondria

D Ardail et al. FEBS Lett. .
Free article

Abstract

The free ceramide content of rat liver mitochondria was found to be 1.7 nmol/mg protein and outer membranes contained a three-fold higher concentration than inner membranes. The mitochondrial content in neutral glycolipids was 0.6 nmol/mg protein. The long-chain bases found in free ceramides were d18:1 sphingosine, d18:0 3-ketosphinganine and t21:1 phytosphingosine in increasing order. In contrast, 3-ketosphinganine was the only base of glucosylceramide and lactosylceramide of inner membranes, whereas d18:1 sphingosine was the major long-chain base of glucosylceramide of outer membranes.

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