Rotor speed dependent sedimentation of circular and linear DNA
- PMID: 111726
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Rotor speed dependent sedimentation of circular and linear DNA
Biophys Chem.
1979 May.
Abstract
The sedimentation rate of large, linear DNA molecules has been shown to be rotor speed dependent (Rubenstein and Leighton, Biophys. Chem. 1 (1974)). In this communication we report the first studies designed to measure the rotor speed effect with a homogenous, linear viral DNA larger than bacteriophage T2 DNA. We also report the first studies using a homogenous, circular episomal DNA of known molecular weight. For this circular DNA a small rotor speed effect, previously unsuspected, was discovered.
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