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. 1979 May;9(4):375-81.

Rotor speed dependent sedimentation of circular and linear DNA

E J Ralston, V N Schumaker
  • PMID: 111726

Rotor speed dependent sedimentation of circular and linear DNA

E J Ralston et al. Biophys Chem. 1979 May.

Abstract

The sedimentation rate of large, linear DNA molecules has been shown to be rotor speed dependent (Rubenstein and Leighton, Biophys. Chem. 1 (1974)). In this communication we report the first studies designed to measure the rotor speed effect with a homogenous, linear viral DNA larger than bacteriophage T2 DNA. We also report the first studies using a homogenous, circular episomal DNA of known molecular weight. For this circular DNA a small rotor speed effect, previously unsuspected, was discovered.

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