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. 1976 Aug;12(8):541-53.
doi: 10.1007/BF02797437.

Rat liver cells in culture: effect of storage, long-term culture, and transformation on some enzyme levels

J B Idoine, J M Elliott, M J Wilson, E K Weisburger

Rat liver cells in culture: effect of storage, long-term culture, and transformation on some enzyme levels

J B Idoine et al. In Vitro. 1976 Aug.

Abstract

Aryl hydrocarbon hydroxylase (AHH) and tyrosine aminotransferase (TAT) activities were determined in rat liver cell lines after frozen storage, long-term culture, and transformation in vitro. Levels of AHH activity after 17 months in frozen storage were comparable to levels prior to freezing. During long-term culture the AHH levels of the cell lines tended to decrease. Transformed lines had variable levels of AHH activity. Cell lines retained measurable TAT activity following long-term culture and frozen storage. TAT activity of transformed cells was comparable to that of normal lines. Prolonged frozen storage did not induce transformation up to one year.

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