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. 2001 Mar;69(3):1914-6.
doi: 10.1128/IAI.69.3.1914-1916.2001.

Regulation of matrix metalloproteinase expression in endothelial cells by heat-inactivated Streptococcus pneumoniae

U Michel  1 , R Zobotke, M Mäder, R Nau
Affiliations

Regulation of matrix metalloproteinase expression in endothelial cells by heat-inactivated Streptococcus pneumoniae

U Michel et al. Infect Immun. 2001 Mar.

Abstract

Matrix metalloproteinases (MMPs) may contribute to an impaired endothelial layer in several diseases. We examined the effect of heat-inactivated Streptococcus pneumoniae R6 on MMP-2 and MMP-9 release by cultured aortic and brain capillary endothelial cells. Treatment with heat-inactivated S. pneumoniae caused an increased release of MMP-2 by both cell types.

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Figures

FIG. 1
FIG. 1
Zymograms of cell culture medium derived from AEC and BMVEC after 24 h of challenge with either 10 nM PMA (lane 2) or 1010 CFU of heat-inactivated S. pneumoniae R6 per ml (lane 3) or without treatment (lane 1). There is an increase in the level of MMP-2 as a result of the S. pneumoniae R6 challenge in supernatants of AEC and BMVEC and of MMP-9 by PMA in AEC culture medium. M, molecular mass markers (Rainbow coloured protein weight marker [Amersham, Little Chalfont, United Kingdom]) in kilodaltons.
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