Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
Review
. 2001 Feb;23(2):191-8.
doi: 10.1006/meth.2000.1120.

Discovery and analysis of antisense oligonucleotide activity in cell culture

B F Baker  1 , T P Condon, E Koller, R A McKay, A M Siwkowski, T A Vickers, B P Monia
Affiliations
Review

Discovery and analysis of antisense oligonucleotide activity in cell culture

B F Baker et al. Methods. 2001 Feb.

Abstract

In the past decade antisense oligonucleotides (ASOs) have proven to be a useful tool for dissection of gene function in molecular cell biology (Koller, E., Gaarde, W. A., and Monia, B. P. (2000) Trends Pharm. Sci., 21, 142-148), and validation of gene targets in animal models (Crooke, S. T. (1998) Biotechnol. Gen. Eng. Rev. 15, 121-157), as well as a means for therapeutic treatment of human diseases (Bennett, C. F. (1999) Exp. Opin. Invest. Drugs 8, 237-253). An important step toward usage of ASOs in the described applications is identification of an active ASO. This article describes the underlying basis and means for achieving this goal in cell culture.

PubMed Disclaimer

LinkOut - more resources