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. 2001 Mar;45(3):973-5.
doi: 10.1128/AAC.45.3.973-975.2001.

Restoration of vancomycin susceptibility in Enterococcus faecalis by antiresistance determinant gene transfer

C Torres Viera  1 , S Tsiodras, H S Gold, E P Coakley, C Wennersten, G M Eliopoulos, R C Moellering Jr, R T Inouye
Affiliations

Restoration of vancomycin susceptibility in Enterococcus faecalis by antiresistance determinant gene transfer

C Torres Viera et al. Antimicrob Agents Chemother. 2001 Mar.

Abstract

We assessed the ability of gene transfer to reverse vancomycin resistance in class A (VanA) glycopeptide-resistant Enterococcus faecalis. Recombinant shuttle vectors containing a vanH promoter-vanA antisense gene cassette fully restored vancomycin susceptibility through a combined transcriptional activator binding domain decoy and inducible vanA antisense RNA effect.

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Figures

FIG. 1
FIG. 1
Proposed dual mechanism of action of the pAM401-vanH promoter-vanA antisense recombinant shuttle vector. The vancomycin susceptibility of a target VanA E. faecalis strain is enhanced through (A) the binding and sequestration of phosphorylated VanR from the native vanH promoter (vanHP) (shaded), thereby inhibiting the induction of the vanHAX genes, and (B) the phosphorylated-VanR-induced vanA antisense RNA effect.
See this image and copyright information in PMC

References

    1. Arthur M, Courvalin P. Genetics and mechanisms of glycopeptide resistance in enterococci. Antimicrob Agents Chemother. 1993;37:1563–1571. - PMC - PubMed
    1. Arthur M, Depardieu F, Courvalin P. Regulated interactions between partner and non-partner sensors and response regulators that control glycopeptide resistance gene expression in enterococci. Microbiology. 1999;145:1849–1858. - PubMed
    1. Arthur M, Depardieu F, Molinas C, Reynolds P, Courvalin P. The vanZ gene of Tn1546 from Enterococcus faecium BM4147 confers resistance to teicoplanin. Gene. 1995;154:87–92. - PubMed
    1. Arthur M, Molinas C, Courvalin P. The VanS-VanR two-component regulatory system controls synthesis of depsipeptide peptidoglycan precursors in Enterococcus faecium BM4147. J Bacteriol. 1992;174:2582–2591. - PMC - PubMed
    1. Arthur M, Molinas C, Depardieu F, Courvalin P. Characterization of Tn1546, a Tn3-related transposon conferring glycopeptide resistance by synthesis of depsipeptide peptidoglycan precursors in Enterococcus faecium BM4147. J Bacteriol. 1993;175:117–127. - PMC - PubMed

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