Dissociation of MNSs antigens using sodium deoxycholate fraction of erythrocyte membranes

Abstract

A dissociation of the MNSs blood group system has been demonstrated by sodium deoxycholate fractionation of erythrocyte membrane preparations. The MN antigens were localized in a fraction that sedimented at 30,000 g while the Ss antigens remained soluble after 100,000 g centrifugation for donors who were heterozygous at the MN locus. In contrast, fractions derived from erythrocytes of 'homozygous' M or N donors showed M or N activity in both the 30,000 g pellet and 100,000 g supernatant and Ss remained in the 100,000 g supernatant.