Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2000 Dec;18(4):347-55.
doi: 10.1023/a:1026785228634.

Lanthanide induced residual dipolar couplings for the conformational investigation of peripheral 15NH2 moieties

I Bertini  1 , I C Felli, C Luchinat
Affiliations

Lanthanide induced residual dipolar couplings for the conformational investigation of peripheral 15NH2 moieties

I Bertini et al. J Biomol NMR. 2000 Dec.

Abstract

The Ca2 calbindin protein in which one calcium has been substituted with Ce(III), Yb(III) and Dy(III) displays substantial alignment in high magnetic fields due to the high anisotropy of the metal magnetic susceptibility. This property has allowed the measurement of residual dipolar coupling contributions to 1J(HN) and 2J(HH) couplings of asparagine and glutamine NH2 moieties. Such data have been used to aid structural characterization of these groups. The exploitation of auto-orientation of magnetic anisotropic metalloproteins represents a step ahead in the investigation of the conformational space of peripheral residues that are not fixed by the protein folding.

PubMed Disclaimer

References

    1. Biochemistry. 1999 Jul 13;38(28):9013-22 - PubMed
    1. J Mol Biol. 1997 Oct 17;273(1):283-98 - PubMed
    1. J Magn Reson. 1999 Aug;139(2):273-80 - PubMed
    1. Nat Struct Biol. 1997 Apr;4(4):292-7 - PubMed
    1. Eur J Biochem. 1990 Jan 26;187(2):455-60 - PubMed

Publication types

MeSH terms

LinkOut - more resources