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. 1975 Jan 6;378(1):73-9.
doi: 10.1016/0005-2787(75)90138-0.

Globin mRNA from rabbit reticulocyte membrane-bound ribosomes

B A Brennessel, J Goldstein

Globin mRNA from rabbit reticulocyte membrane-bound ribosomes

B A Brennessel et al. Biochim Biophys Acta. .

Abstract

Messenger RNA has been isolated from both free and membrane-bound rabbit reticulocyte ribosomes. Membrane-bound ribosomes, which constitute less than 10% of total cellular ribosomes, are released from the membrane by deoxycholate but remain associated with the membrane fraction following treatment with 0.5 M KC1. The major messenger RNA species isolated from either free or bound ribosomes is globin messenger. Both alpha- and beta-globin chains are synthesized when the isolated messenger RNA from either free or membrane-bound reticulocyte ribosomes is added to a Krebs ascites cell-free system.

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