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Review
. 2000 Nov;20(6):391-9.
doi: 10.1023/a:1026403724413.

Immunofluorescence analysis of T-cell responses in health and disease

H T Maecker  1 , V C Maino, L J Picker
Affiliations
Review

Immunofluorescence analysis of T-cell responses in health and disease

H T Maecker et al. J Clin Immunol. 2000 Nov.

Abstract

The use of flow cytometry to study the functional responses of T cells by immunofluorescent staining for intracellular cytokines and other markers is a growing field of clinical interest. In this article, we describe methods for the rapid evaluation of T-cell responses to mitogens and specific antigens and explore how these assays might be valuable in various clinical settings.

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