Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
Comment
. 2001 Jan 16;98(2):401-3.
doi: 10.1073/pnas.98.2.401.

Transmitochondrial mice: proof of principle and promises

M Hirano  1
Affiliations
Comment

Transmitochondrial mice: proof of principle and promises

M Hirano. Proc Natl Acad Sci U S A. .
No abstract available

PubMed Disclaimer

Figures

Figure 1
Figure 1
Experimental strategy used by Sligh et al. (5) to produce two types of transmitochondrial mice. To produce transmitochondrial mice with NZB mtDNA (indicated by gray ovals), synaptosomes from NZB mice were fused with ρ0 cells (mitochondria lacking DNA; indicated by empty ovals) to generate cybrids harboring NZB mitochondria. These cybrids were enucleated and fused with ES cells depleted of mitochondria with rhodamine-6G (R-6G). The resulting ES cybrids containing NZB mitochondria were injected into C57BL/6J blastocysts to produce chimeric and ultimately transmitochondrial mice (NZB mtDNA with C57BL/6J nDNA). To produce mice with heteroplasmic mtDNA mutation conferring CAPR (mitochondria with mutation; indicated by black ovals), cybrids harboring the mutation were enucleated and fused with R-6G-treated ES cells.
See this image and copyright information in PMC

Comment on

References

    1. DiMauro S, Andreu A. Brain Pathol. 2000;10:431–441. - PMC - PubMed
    1. King M P, Attardi G. Science. 1989;246:500–503. - PubMed
    1. Larsson N-G, Wang J, Wilhelmsson H, Oldfors A, Rustin P, Lewandoski M, Barsh G S, Clayton D A. Nat Genet. 1998;18:231–236. - PubMed
    1. Wallace D C. Science. 1999;283:1482–1488. - PubMed
    1. Sligh J E, Levy S E, Waymire K G, Allard P, Dillehay D L, Nusinowitz S, Heckenlively J R, MacGregor G R, Wallace D C. Proc Natl Acad Sci USA. 2000;97:14461–14466. . (First Published December 5, 2000; 10.1073/pnas.250491597) - PMC - PubMed

MeSH terms

LinkOut - more resources