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. 2001 Feb;12(2):141-9.
doi: 10.1007/s003350010248.

Fine-mapping and construction of a bovine contig spanning the ovine callipyge locus

T L Shay  1 , S Berghmans, K Segers, S Meyers, J E Beever, J E Womack, M Georges, C Charlier, N E Cockett
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Fine-mapping and construction of a bovine contig spanning the ovine callipyge locus

T L Shay et al. Mamm Genome. 2001 Feb.

Abstract

The callipyge (CLPG) gene was fine-mapped by linkage analysis to a 4.6-cM chromosome interval on distal ovine OAR18q, flanked by microsatellite markers IDVGA30 and OY3. The OAR18q linkage map and human HSA14q transcript map were aligned by genotyping two bovine-hamster whole-genome radiation hybrid panels with the microsatellite markers, as well as with sequences corresponding to HSA 14q genes. Using Type I loci mapping to the IDVGA30-OY3 interval as anchor points, we have constructed a 1.4-Mb bovine BAC contig containing the IDVGA30-OY3 interval. We demonstrate that the IDVGA30-OY3 interval spans approximately 770 kb and contains at least four genes: YY1, WARS, DLK1, and GTL2.

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