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. 1975 Feb;2(2):165-78.
doi: 10.1093/nar/2.2.165.

Absence of mRNA for casein in free polysomes of lactating ewe mammary gland

L M Houdebine, P Gaye
Free PMC article

Absence of mRNA for casein in free polysomes of lactating ewe mammary gland

L M Houdebine et al. Nucleic Acids Res. 1975 Feb.
Free PMC article

Abstract

Total polysomal RNA or poly A- containing RNA extracted from free polysomes of lactating ewe mammary gland was unable to direct a significant synthesis of alpha-s- and beta-casein. Polyacrylamide gel electrophoresis of poly A- containing RNA revealed that the two major peaks of mRNA isolated from membrane-bound polysomes were not observed in mRNA isolated from free polysomes. Purified 125-I anti-alpha-s-casein could bind significantly to membrane-bound polysomes only. These data suggest that casein synthesis occurs almost exclusively on membrane-bound polysomes and that this specificity may be attributed to the presence of casein mRNAs on these polysomes and their absence from free polysomes.

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References

    1. Biochem Biophys Res Commun. 1973 Mar 5;51(1):81-7 - PubMed
    1. Biochem Biophys Res Commun. 1973 May 1;52(1):79-87 - PubMed
    1. Biochem Biophys Res Commun. 1972 Nov 1;49(3):713-9 - PubMed
    1. Biochim Biophys Acta. 1972 Mar 24;262(3):371-80 - PubMed
    1. J Mol Biol. 1972 Apr 14;65(3):413-22 - PubMed