Anatomical and histological changes in the oviducts of Japanese quail, Coturnix japonica, after embryonic exposure to ethynyloestradiol

Abstract

Oestrogen is needed for normal oviductal development in female birds, but excessive early exposure to oestrogen can cause oviductal abnormalities and impair egg-laying ability. In this study, the anatomical and histological effects of in ovo exposure to the synthetic oestrogen ethynyloestradiol on the oviducts of immature and adult female Japanese quail, Coturnix japonica, were investigated. A series of abnormalities was observed after injection of ethynyloestradiol (2 or 20 ng g(-1) egg) into the yolk on day 3 of incubation. Ethynyloestradiol induced precocious differentiation of the luminal epithelium and tubular glands in immature chicks. Right-side oviduct retention occurred at all the ages studied, whereas certain other effects were not evident until sexual maturity. The left oviduct was reduced in size and tubular gland density in the uterus (shell gland) was reduced in sexually mature birds that had been treated with ethynyloestradiol. The utero-vaginal junction was longer than in control birds and had a higher tubular gland density. The epithelial cells in the magnum were taller in birds treated with ethynyloestradiol. Embryonic exposure to the environmental contaminant ethynyloestradiol may cause persisting structural malformations in oviducts of quails, which can impair fertility. As oviductal malformations are indicative of embryonic exposure to exogenous oestrogen, they are potentially useful as biomarkers of xenooestrogen exposure in wild bird populations.