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. 2001 Feb 15;409(6822):942-3.
doi: 10.1038/35057165.

The physical maps for sequencing human chromosomes 1, 6, 9, 10, 13, 20 and X

D R Bentley  1 , P Deloukas, A Dunham, L French, S G Gregory, S J Humphray, A J Mungall, M T Ross, N P Carter, I Dunham, C E Scott, K J Ashcroft, A L Atkinson, K Aubin, D M Beare, G Bethel, N Brady, J C Brook, D C Burford, W D Burrill, C Burrows, A P Butler, C Carder, J J Catanese, C M Clee, S M Clegg, V Cobley, A J Coffey, C G Cole, J E Collins, J S Conquer, R A Cooper, K M Culley, E Dawson, F L Dearden, R M Durbin, P J de Jong, P D Dhami, M E Earthrowl, C A Edwards, R S Evans, C J Gillson, J Ghori, L Green, R Gwilliam, K S Halls, S Hammond, G L Harper, R W Heathcott, J L Holden, E Holloway, B L Hopkins, P J Howard, G R Howell, E J Huckle, J Hughes, P J Hunt, S E Hunt, M Izmajlowicz, C A Jones, S S Joseph, G Laird, C F Langford, M H Lehvaslaiho, M A Leversha, O T McCann, L M McDonald, J McDowall, G L Maslen, D Mistry, N K Moschonas, V Neocleous, D M Pearson, K J Phillips, K M Porter, S R Prathalingam, Y H Ramsey, S A Ranby, C M Rice, J Rogers, L J Rogers, T Sarafidou, D J Scott, G J Sharp, C J Shaw-Smith, L J Smink, C Soderlund, E C Sotheran, H E Steingruber, J E Sulston, A Taylor, R G Taylor, A A Thorpe, E Tinsley, G L Warry, A Whittaker, P Whittaker, S H Williams, T E Wilmer, R Wooster, C L Wright
Affiliations

The physical maps for sequencing human chromosomes 1, 6, 9, 10, 13, 20 and X

D R Bentley et al. Nature. .

Abstract

We constructed maps for eight chromosomes (1, 6, 9, 10, 13, 20, X and (previously) 22), representing one-third of the genome, by building landmark maps, isolating bacterial clones and assembling contigs. By this approach, we could establish the long-range organization of the maps early in the project, and all contig extension, gap closure and problem-solving was simplified by containment within local regions. The maps currently represent more than 94% of the euchromatic (gene-containing) regions of these chromosomes in 176 contigs, and contain 96% of the chromosome-specific markers in the human gene map. By measuring the remaining gaps, we can assess chromosome length and coverage in sequenced clones.

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