Phosphatidylcholine inhibits and lysophosphatidylcholine enhances the lymphatic absorption of alpha-tocopherol in adult rats

Abstract

This study was conducted to compare the effects of enterally infused phosphatidylcholine (PC) and lysophosphatidylcholine (lysoPC) on the lymphatic absorption of alpha-tocopherol (alphaTP) in male rats. In expt. 1, bile-diverted rats with mesenteric lymph cannulas were infused at 3.0 mL/h for 8 h with a lipid emulsion containing 5.0 micromol alphaTP, 565 micromol 14C-triolein (14C-OA) and 396 micromol Na+-taurocholate with 80 micromol 1,2-dipalmitoyl PC (DPPC) or 1,2-dilinoleoyl PC (DLPC) or without PC (NoPC) in 24 mL phosphate-buffered saline (pH 6.6). In expt. 2, the effects of 1,2-dioleoyl PC (DOPC) and 1-oleoyl-2-hydroxy-PC (lysoPC) on alphaTP and 14C-cholesterol absorption were compared in rats with lymph cannulas. When DPPC or DLPC was infused, the lymphatic absorption of alphaTP was lowered drastically. The cumulative absorptions of alphaTP in rats infused with DPPC and DLPC were 45 and 52%, respectively, of the control values (NoPC). No significant difference was noted between the PC groups. In contrast, the absorption of 14C-OA was increased by 42 to 43% in rats infused with DPPC or DLPC compared with that in NoPC rats. Phospholipid outputs also were significantly higher in DPPC (34.0 +/- 5.5 micromol /8 h) and DLPC (32.4 +/- 2.4 micromol /8 h) rats than in NoPC rats (21.2 +/- 4.2 micromol /8 h). When lysoPC was infused, the absorptions of alphaTP and 14C-cholesterol were increased markedly compared with those for DOPC, with no significant difference in PL output between groups infused with DOPC and lysoPC. These observations provide clear evidence that PC present in a lipid emulsion inhibits alphaTP absorption, whereas it enhances the absorption of fat. The data also demonstrate that lysoPC simultaneously increases the absorption of alphaTP and cholesterol. The findings indicate that luminal PC inhibits the absorption of alphaTP and that hydrolysis of PC is critical to improving the intestinal absorption of the vitamin.