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Review
. 2001 Mar 13;98(6):2950-2.
doi: 10.1073/pnas.061032398.

Right on target: novel approaches for the direct visualization of CD1-specific T cell responses

M Kronenberg  1 , O Naidenko, F Koning
Affiliations
Review

Right on target: novel approaches for the direct visualization of CD1-specific T cell responses

M Kronenberg et al. Proc Natl Acad Sci U S A. .
No abstract available

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Figures

Figure 1
Figure 1
Tetramer staining of human NK T cells. Human peripheral blood mononuclear cells were labeled with 5,6-carboxyfluorescein diacetate succinimidyl ester (CFSE) and cultured subsequently in RPMI medium 1640 supplemented with 10% (vol/vol) pooled human serum/100 units rIL-2/100 ng/ml αGalCer. After 4 days, cells were harvested and stained with a phycoerythrin-labeled Vβ11-specific antibody and a tricolor-labeled αGalCer–CD1 tetramer and analyzed on a FACScan (fluorescence-activated cell sorter). Both the Vβ11-specific antibody (data not shown) and the αGalCer–CD1 tetramer stained a similar population of cells, the majority of which had divided. The percentage of Vβ11/tetramer-positive cells at the start of the culture was 0.6%; after 7 days, it was 20% (data not shown).
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