Inhibition of macromolecular synthesis in L1210 mouse leukemia ascites cells by hycanthone

Abstract

Hycanthone inhibits macromolecular synthesis in L1210 cells. At the same concentrations of hycanthone, DNA synthesis is inhibited to a greater extent than the synthesis of RNA. There is an inhibition by the drug of the synthesis of ribosomal precursor RNA; the synthesis of other types of RNA including heterogeneous and small-molecular-weight, also appears to be inhibited. Maturation of 45-S RNA does not seem to be affected, and there is a partial reversal of inhibition of both DNA and RNA synthesis by washing the cells free of hycanthone.