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. 2000 May-Jun;43(3):183-90.
doi: 10.1016/s1056-8719(00)00085-x.

A transition metal enhanced luminol chemiluminescence in the presence of a chelator

I Parejo  1 , C Petrakis, P Kefalas
Affiliations

A transition metal enhanced luminol chemiluminescence in the presence of a chelator

I Parejo et al. J Pharmacol Toxicol Methods. 2000 May-Jun.

Abstract

We have investigated the chemiluminescence signal of luminol and hydrogen peroxide in the presence of a transition metal (Co(II), Cu(I), Fe(II), Fe(III)) and of a chelator (EDTA, citric acid) in pH 8.5, 9 and 10 borate buffer solutions. We observed that the chemiluminescence intensities of these systems reached a plateau, where they remained stable for a period of 2-30 s. We also observed linearity between the intensity of chemiluminescence and the hydrogen peroxide concentration. The combination of Co(II) and EDTA at pH 9 was found to give the optimum signal with reference to time stability, intensity and reproducibility. Thus, compared to previous chemiluminescence applications, the present results permit us to propose a simple, enzyme-free and time-independent technique for the detection and quantification of hydrogen peroxide.

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