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Review
. 2001 Apr;5(2):137-43.
doi: 10.1016/s1367-5931(00)00182-4.

Improved biocatalysts by directed evolution and rational protein design

U T Bornscheuer  1 , M Pohl
Affiliations
Review

Improved biocatalysts by directed evolution and rational protein design

U T Bornscheuer et al. Curr Opin Chem Biol. 2001 Apr.

Abstract

The efficient application of biocatalysts requires the availability of suitable enzymes with high activity and stability under process conditions, desired substrate selectivity and high enantioselectivity. However, wild-type enzymes often need to be optimized to fulfill these requirements. Two rather contradictory tools can be used on a molecular level to create tailor-made biocatalysts: directed evolution and rational protein design.

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