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. 2001 Apr;67(4):1581-6.
doi: 10.1128/AEM.67.4.1581-1586.2001.

Genomic relatedness within five common Finnish Campylobacter jejuni pulsed-field gel electrophoresis genotypes studied by amplified fragment length polymorphism analysis, ribotyping, and serotyping

M L Hänninen  1 , P Perko-Mäkelä, H Rautelin, B Duim, J A Wagenaar
Affiliations

Genomic relatedness within five common Finnish Campylobacter jejuni pulsed-field gel electrophoresis genotypes studied by amplified fragment length polymorphism analysis, ribotyping, and serotyping

M L Hänninen et al. Appl Environ Microbiol. 2001 Apr.

Abstract

Thirty-five Finnish Campylobacter jejuni strains with five SmaI/SacII pulsed-field gel electrophoresis (PFGE) genotypes selected among human and chicken isolates from 1997 and 1998 were used for comparison of their PFGE patterns, amplified fragment length polymorphism (AFLP) patterns, HaeIII ribotypes, and heat-stable (HS) serotypes. The discriminatory power of PFGE, AFLP, and ribotyping with HaeIII were shown to be at the same level for this selected set of strains, and these methods assigned the strains into the same groups. The PFGE and AFLP patterns within a genotype were highly similar, indicating genetic relatedness. The same HS serotypes were distributed among different genotypes, and different serotypes were identified within one genotype. HS serotype 12 was only associated with the combined genotype G1 (PFGE-AFLP-ribotype). These studies using polyphasic genotyping methods suggested that common Finnish C. jejuni genotypes form genetic lineages which colonize both humans and chickens.

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Figures

FIG. 1
FIG. 1
SmaI PFGE patterns of C. jejuni strains. Lanes 1 to 5, SmaI pattern I, strains 4772 (lane 1), 25A (lane 2), 5483 (lane 3), 40A (lane 4), and 28A (lane 5); lanes 6 to 9, pattern VII, strains 5862 (lane 6), FB5241 (lane 7), 4859 (lane 8), and FB5519 (lane 9); lanes 10 and 11, pattern T101, strain FB6271 (lane 10) and 4180 (lane 11); lanes 12 and 13, pattern IV, strains FB287 (lane 12) and strain 35A (lane 13); mw, molecular size marker.
FIG. 2
FIG. 2
SacII patterns of same strains as in Fig. 1. Lanes 1 and 4, pattern K; lane 2, pattern Kc; lane 3, pattern Ka; lane 5, pattern Kb; lane 6, pattern VII; lane 7, pattern VIa; lane 8, pattern VIb; lane 9, pattern VIc; lane 10, pattern T101a; lane 11, pattern T101b; lanes 12 and 13, pattern IV. mw, molecular size marker.
FIG. 3
FIG. 3
AFLP patterns of 35 C. jejuni strains selected for the study.
FIG. 4
FIG. 4
HaeIII ribopattern types of C. jejuni strains selected for studies. Lane 1, strain 4593, type A; lane 2, strain 4772, type A; lane 3, strain 25A, type B; lane 4, strain 5483, type Aa; lane 5, strain BR100, type C; lane 6, strain FB8164, type Ca; lane 7, strain FB5241, type D; lane 8, strain BR170, type D; lane 9, strain 5862, type E; lane 10, strain FB4877, type D; lane 11, strain 5259, type D; lane 12, FB6271, type F; lane 13, strain FB456, type F; lane 14, strain FB4180, type Fa; lane 15, molecular size marker (2.0, 2.3, 4.3, 6.5, 9.4, and 23 kb).
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