Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2001 Apr;39(4):1436-42.
doi: 10.1128/JCM.39.4.1436-1442.2001.

Application and evaluation of the interlaboratory reproducibility of tRNA intergenic length polymorphism analysis (tDNA-PCR) for identification of Streptococcus species

M Baele  1 , V Storms, F Haesebrouck, L A Devriese, M Gillis, G Verschraegen, T de Baere, M Vaneechoutte
Affiliations

Application and evaluation of the interlaboratory reproducibility of tRNA intergenic length polymorphism analysis (tDNA-PCR) for identification of Streptococcus species

M Baele et al. J Clin Microbiol. 2001 Apr.

Abstract

The discriminatory power, speed, and interlaboratory reproducibility of tRNA intergenic length polymorphism analysis (tDNA-PCR) combined with capillary electrophoresis was evaluated for the identification of streptococci. This method was carried out in three different laboratories under highly standardized conditions for 54 strains belonging to 18 different species. It was concluded that interlaboratory reproducibility of tDNA fingerprints produced by means of capillary electrophoresis was sufficiently high to permit the exchange between different laboratories and the construction of common libraries which can be consulted for comparison with fingerprints obtained independently in separate laboratories. In a second step, 17 other species were included in the study and examined in one of the participating laboratories. All Streptococcus species studied, except S. mitis, S. oralis, S. parasanguinis, S. pneumoniae, S. thermophilus, and S. vestibularis, showed distinguishable tDNA fingerprints. A database of well-characterized strains was constructed to enable computer-aided identification of unknown streptococcal isolates.

PubMed Disclaimer

Figures

FIG. 1
FIG. 1
Dendrogram obtained after similarity calculations between the tDNA fingerprints of streptococci. The bar represents a distance of 10%.
See this image and copyright information in PMC

References

    1. Baele M, Baele P, Vaneechoutte M, Storms V, Butaye P, Devriese L A, Verschraegen G, Gillis M, Haesebrouck F. Application of tDNA-PCR for the identification of enterococci. J Clin Microbiol. 2000;38:4201–4207. - PMC - PubMed
    1. Bentley R W, Leigh J A, Collins M D. Intrageneric structure of Streptococcus based on comparative analysis of small-subunit rRNA sequences. Int J Syst Bacteriol. 1991;41:487–494. - PubMed
    1. Degheldre Y, Vandamme P, Goossens H, Struelens M. Identification of clinically relevant viridans streptococci by analysis of transfer DNA intergenic spacer length polymorphism. Int J Syst Evol Microbiol. 1999;49:1591–1598. - PubMed
    1. Ehrenstein B, Bernards A T, Dijkshoorn L, Gerner S P, Towner K J, Bouvet P J M, Daschner F D, Grundmann H. Acinetobacter species identification by using tRNA spacer fingerprinting. J Clin Microbiol. 1996;34:2414–2420. - PMC - PubMed
    1. Farrow J A, Collins M D. Taxonomic studies on streptococci of serological groups C, G and L and possibly related taxa. Syst Appl Microbiol. 1984;5:483–493.

Publication types

MeSH terms

LinkOut - more resources