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. 2001 Apr;39(4):1600-3.
doi: 10.1128/JCM.39.4.1600-1603.2001.

Typing of Candida albicans isolates by sequence analysis of the cytochrome b gene and differentiation from Candida stellatoidea

S K Biswas  1 , K Yokoyama, L Wang, K Nishimura, M Miyaji
Affiliations

Typing of Candida albicans isolates by sequence analysis of the cytochrome b gene and differentiation from Candida stellatoidea

S K Biswas et al. J Clin Microbiol. 2001 Apr.

Abstract

Including type strains, mitochondrial cytochrome b genes of 32 strains of Candida albicans and 6 strains of Candida stellatoidea, presently treated as a synonym for C. albicans, were partially sequenced. Analysis of 396-bp nucleotide sequences of the strains under investigation divided C. albicans isolates into three types: type I, type II, and type III; however, strains of C. stellatoidea represented distinct type IV isolates. Deduced amino acid sequences of type I, type II, and type III were identical and differed from that of type IV by one amino acid. Genotypes (rDNA type) of the test strains were also checked. Cytochrome b typing did not correlate with genotyping, and different genotypes occurred for one cytochrome b type. This study shows that cytochrome b gene sequences are useful for analyzing the genetic relatedness of C. albicans isolates and effective for differentiating C. stellatoidea from C. albicans.

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Figures

FIG. 1
FIG. 1
Comparison of the nucleotide sequences of cytochrome b genes of various types of C. albicans isolates. Dots indicate that the nucleotides are the same as those of C. albicans type I.
FIG. 2
FIG. 2
Comparison of deduced amino acid sequences of cytochrome b genes of various types of C. albicans isolates. Dots indicate that the amino acids are the same as those of C. albicans type I.
FIG. 3
FIG. 3
UPGMA-based trees representing the relationships of various types of C. albicans isolates, based on nucleotide (a) and deduced amino acid (b) sequences of the cytochrome b gene. The DDBJ accession no. of the C. dubliniensis type strain is AB044913 (26). Bars represent the numbers of nucleotide and amino acid substitutions per nucleotide and amino acid site.
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