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Comparative Study
. 2001 Apr;39(4):1626-9.
doi: 10.1128/JCM.39.4.1626-1629.2001.

Nucleic acid sequence-based amplification of Aspergillus RNA in blood samples

J Loeffler  1 , H Hebart, P Cox, N Flues, U Schumacher, H Einsele
Affiliations
Comparative Study

Nucleic acid sequence-based amplification of Aspergillus RNA in blood samples

J Loeffler et al. J Clin Microbiol. 2001 Apr.

Abstract

Nucleic acid sequence-based amplification (NASBA), an isothermal amplification technique, was established and evaluated for the detection of Aspergillus RNA and compared with a previously published, well-defined real-time PCR assay amplifying a region of the Aspergillus 18S rRNA gene. NASBA showed a lower detection limit of 1 CFU and detected RNA from five different clinically relevant Aspergillus species, including Aspergillus fumigatus. All 77 blood samples tested by PCR and NASBA showed identical results in both assays. Results with the NASBA technique were obtained within 6 h. Thus, the NASBA technique provided a valuable tool for sensitive, specific, fast, and reliable detection of Aspergillus RNA with potential for routine diagnosis, including the possibility to test the viability of cells.

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Figures

FIG. 1
FIG. 1
Influence of time between sampling and extraction on detectable RNA level.
See this image and copyright information in PMC

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