Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2001 May;69(5):3410-2.
doi: 10.1128/IAI.69.5.3410-3412.2001.

Passive immunization with melanin-binding monoclonal antibodies prolongs survival of mice with lethal Cryptococcus neoformans infection

A L Rosas  1 , J D Nosanchuk, A Casadevall
Affiliations

Passive immunization with melanin-binding monoclonal antibodies prolongs survival of mice with lethal Cryptococcus neoformans infection

A L Rosas et al. Infect Immun. 2001 May.

Abstract

Passive immunization with monoclonal antibodies (MAbs) to melanin prolonged the survival of and reduced the fungal burden in Cryptococcus neoformans-infected mice in comparison to controls. MAbs to melanin reduced the growth rate of in vitro-melanized C. neoformans cells, suggesting a new mechanism of antibody-mediated protection.

PubMed Disclaimer

Figures

FIG. 1
FIG. 1
(A) Passive immunization with MAb 6D2 or 11B11 to melanin prolongs the survival of mice infected with C. neoformans strain 24067 in comparison to mice given NSO ascites (10 mice per group). (B) Passive immunization with MAb 6D2 or 11B11 prolongs the survival of mice infected with C. neoformans strain H99 in comparison to mice given MAb 5B11 with specificity for mycobacterial lipoarabinomannan (10 mice per group).
FIG. 2
FIG. 2
Incubation of MAbs to melanin or melanin-binding peptide reduces the growth rate of in vitro-melanized C. neoformans cells in comparison to growth with MAb 5C11 or a control peptide. Data points represent the mean of measurements for two wells for each concentration from a single experiment.
See this image and copyright information in PMC

References

    1. Casadevall A, Rosas Á, Nosanchuk J D. Melanin and virulence in Cryptococcus neoformans. Curr Opn Microbiol. 2000;3:354–358. - PubMed
    1. Chen L, Blank E S, Casadevall A. Extracellular proteinase activity of Cryptococcus neoformans. Clin Diagn Lab Immunol. 1996;3:570–574. - PMC - PubMed
    1. Glatman-Freedman A, Martin J M, Rifka P F, Bloom B R, Casadevall A. Monoclonal antibodies to surface antigens of Mycobacterium tuberculosis and their use in a modified enzyme-linked immunosorbent spot assay for detection of mycobacteria. J Clin Microbiol. 1996;34:2795–2802. - PMC - PubMed
    1. Kozel T R. Virulence factors of Cryptococcus neoformans. Trends Microbiol. 1995;3:295–299. - PubMed
    1. Mitchell T G, Perfect J R. Cryptococcosis in the era of AIDS—100 years after the discovery of Cryptococcus neoformans. Clin Microbiol Rev. 1995;8:515–548. - PMC - PubMed

Publication types

LinkOut - more resources