Screening and search for novel inhibitors of DD-peptidase 64-575
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- PMID: 11293273
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Screening and search for novel inhibitors of DD-peptidase 64-575
Acta Pol Pharm.
2000 Nov.
Abstract
The aim of the work was search for inhibitors of PBPs (penicillin binding proteins, DD-carboxypeptidase/transpeptidase, DD-peptidase). Between 141 tested Streptomyces strains, 25% showed activity of inhibitors production. The inhibitors were produced by selected Streptomyces strains (NIH Culture Collection). The culture supernatants of Streptomyces rimosus B PZH (inhibitor B) and Streptomyces rimosus NRRL 2234 (inhibitor 2234) exhibited the highest inhibition activity. Both inhibitors were purified by the use of: anion exchange chromatography and reversed phase chromatography (HPLC). Inhibitor B is a gamma-lactam compound and inhibitor 2234 is a beta-lactam.
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