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. 1978 Dec;31(6):679-88.
doi: 10.1071/bi9780679.

Transformation of Pseudomonas aeruginosa strain PAO with bacteriophage and plasmid DNA

M I Sinclair, A F Morgan

Transformation of Pseudomonas aeruginosa strain PAO with bacteriophage and plasmid DNA

M I Sinclair et al. Aust J Biol Sci. 1978 Dec.

Abstract

A procedure has been developed which allows transformation of P. aeruginosa strain PAO with plasmid and bacteriophage DNA at a frequency of 10(-6) per recipient cell. The method is similar in outline to that developed for Escherichia coli. It involves growing the recipient cells to 3-5 x 10(8) per ml in nutrient broth, washing the cells with 0.1 M MgCl2, resuspending in 0.175 M CaCl2 for 20 min, exposing to DNA for 1 h and then heat pulsing at 42 degrees C for 1 min. Some plasmid markers are expressed immediately, whereas others require time for phenotypic expression.

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