Review
doi: 10.2302/kjm.50.26.
The development and applications of the bacterial artificial chromosome cloning system
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- PMID: 11296661
- DOI: 10.2302/kjm.50.26
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Review
The development and applications of the bacterial artificial chromosome cloning system
Keio J Med.
2001 Mar.
Free article
Abstract
The development of the Bacterial Artificial Chromosome (BAC) system was driven in part by the Human Genome Project as a means to construct genomic DNA libraries and physical maps for genomic sequencing. The BAC system is based on the well-characterized Escherichia coli F-factor, a low copy plasmid that exists in a supercoiled circular form in host cells. The structural features of the F-factor allow stable maintenance of individual human DNA clones as well as easy manipulation of the cloned DNA. BACs are currently used in a wide array of applications from genome sequencing to gene discovery. This paper describes the key elements in the development of the BAC system and its current notable applications.
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