Arginine decarboxylase transgene expression and analysis of environmental stress tolerance in transgenic rice

Abstract

Arginine decarboxylase (ADC) cDNA from oat (Avena sativa L.) was introduced into rice (Oryza sativa L.) genome by an Agrobacterium-mediated transformation method. Expression of the ADC transgene under the control of an ABA-inducible promoter led to stress-induced upregulation of ADC activity and polyamine accumulation in transgenic rice plants. Second-generation (Rl) transgenic rice plants showed an increase in biomass under salinity-stress conditions, as compared to the non-transformed control plants.