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. 1975;22(1):57-66.

Molecular properties of the inducible lupanine hydroxylase from growing cultures of Pseudomonas lupanini

J Rogoziński
  • PMID: 1130159

Molecular properties of the inducible lupanine hydroxylase from growing cultures of Pseudomonas lupanini

J Rogoziński. Acta Biochim Pol. 1975.

Abstract

An improved method for isolation of lupanine hydroxylase, giving a 450-fold purification, is presented. The molecular weight of the enzyme is about 72 000, and the sedimentation coefficient S20, W 5.05. The enzyme contains a component similar to Pseudomonas cytochromes c. Its oxidation-reduction potential was found to be below + 45 mV.

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