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. 2001 May;45(5):1595-8.
doi: 10.1128/AAC.45.5.1595-1598.2001.

Serotype 19f multiresistant pneumococcal clone harboring two erythromycin resistance determinants (erm(B) and mef(A)) in South Africa

L McGee  1 , K P Klugman, A Wasas, T Capper, A Brink
Affiliations

Serotype 19f multiresistant pneumococcal clone harboring two erythromycin resistance determinants (erm(B) and mef(A)) in South Africa

L McGee et al. Antimicrob Agents Chemother. 2001 May.

Abstract

One hundred eighteen erythromycin-resistant Streptococcus pneumoniae (ERSP) strains (MICs of > or = 0.5 microg/ml) from five laboratories serving the private sector in South Africa were analyzed for the genes encoding resistance to macrolides. Sixty-seven ERSP strains (56.8%) contained the erm(B) gene, and 15 isolates (12.7%) contained the mef(A) gene. Thirty-six isolates (30.5%) harbored both the erm(B) and mef(A) genes and were highly resistant to erythromycin and clindamycin. DNA fingerprinting by BOX-PCR and pulsed-field gel electrophoresis identified 83% of these strains as belonging to a single multiresistant serotype 19F clone.

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Figures

FIG. 1
FIG. 1
Geographic locations of laboratories and distribution of isolates belonging to a serotype 19F clone in South Africa.
FIG. 2
FIG. 2
DNA fingerprint patterns for representative isolates of the 19F multiresistant clone with erm(B) and mef(A) genes by BOX-PCR (a) and PFGE (b). Lanes: 1, R6 (unencapsulated laboratory strain); 2 to 4, isolates PA7062, D1, and J5, respectively; 5 to 7, Spain23F-1, Spain6B-2, and France9v-3, respectively; M, DNA molecular weight marker VI (a) and λ DNA ladder (b) (Roche Diagnostics, Mannheim, Germany).
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