doi: 10.1159/000056888.
Induction and characterization of hypoxanthine-phosphoribosyltransferase (Hprt-) deficient cell lines of Akodon cursor (Rodentia, Sigmodontinae)
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- PMID: 11306816
- DOI: 10.1159/000056888
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Induction and characterization of hypoxanthine-phosphoribosyltransferase (Hprt-) deficient cell lines of Akodon cursor (Rodentia, Sigmodontinae)
Cytogenet Cell Genet.
2001.
Abstract
Hypoxanthine-phosphoribosyltransferase negative (Hprt-) cell lines derived from an Akodon cursor liposarcoma were obtained by induced mutagenesis. All but one Hprt- cell line lacked Hprt mRNA transcripts while one (AKO 3) coded for a truncated protein. Cell fusion and karyotypic analyses showed that one cell line (AKO 1-15) could be successfully used for constructing hybrid panels and allow for a clear identification of human chromosomes in hybrid cells.
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