Dietary antioxidative potential in arteries

Abstract

Many researches show that traditional Indonesian diet is good for physical health. The present study examines the antioxidative, anti-inflammatory and antithrombotic potentials of aqueous extract of tempe (fermented soya-beans) and Curcuma domestica in an experimental photochemical thrombogenesis model using rat femoral artery. A total of 15 male Wistar rats weighing 250 g were used, and divided into three groups: control (group-1, n = 5), animals, treated orally with 0.25 ml aqueous extracts of 50 g tempe (fermented soybean cake) once daily for 7 consecutive days (group-2, n = 5) and animals treated orally with 0.25 ml aqueous extracts of 10 g roots of Curcuma domestica once daily for 5 consecutive days (group-3, n = 5). All animals were anesthetized, and Sn-pyrophosphate and Tc99m solutions were injected intravenously for in vivo red cell radioactive labeling. Femoral arterial occlusion was observed, using a gamma camera. Induction of femoral arterial thrombosis was effected following an endothelial injury by free radicals produced by green light-irradiated Rose Bengal (10 mg/kg). The results showed that in the control group arterial total flow occlusion was seen in 15 min of irradiation. The results of MDA absorbency was 0.3700 +/- 1.7 nmol/ml in control group-1, 0.0520 +/- 0.025 in group-2 (significant p < 0.05 in comparison to control group) and 0.2780 +/- 0.027 in group-3 (non-significant). Interleukin-1alpha plasma level was 14.44 +/- 2.3 in control group-1, 8.93 +/- 2.4 in group-2 (significant p < 0.05), and 6.21 +/- 2.5 in group-3 (significant p < 0.05). Plasma thromboxane B2 plasma level was 20.31 +/- 2.4 in control group-1, 14.32 +/- 2.2 in group-2 (significant p < 0.05), and 19.41 +/- 2.1 in group-3 (significant). This study suggests the potential antioxidative, anti-inflammatory and antithrombotic effect that the dietary aqueous extracts has in rat femoral artery.