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. 1979 Aug 15;35(8):1045-7.
doi: 10.1007/BF01949932.

Effects of calcium-EGTA buffers on active calcium transport in inside-out red cell membrane vesicles

B Sarkadi, A Schubert, G Gárdos

Effects of calcium-EGTA buffers on active calcium transport in inside-out red cell membrane vesicles

B Sarkadi et al. Experientia. .

Abstract

In inside-out red cell membrane vesicles, the free calcium concentration half-maximally stimulating active calcium uptake is about 2 orders of magnitude smaller in a calcium-EGTA buffer than in media containing unbuffered calcium. In calcium-EGTA buffer, the maximum rate of calcium uptake is determined by the total calcium concentration present. A possible model for explaining these findings is presented.

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