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. 2001 May;39(5):1788-90.
doi: 10.1128/JCM.39.5.1788-1790.2001.

Effects of storage and type of blood collection tubes on hepatitis C virus level in whole blood samples

H H Kessler  1 , E Stelzl, R B Raggam, J Haas, F Kirchmeir, K Hegenbarth, E Daghofer, B I Santner, E Marth, R E Stauber
Affiliations

Effects of storage and type of blood collection tubes on hepatitis C virus level in whole blood samples

H H Kessler et al. J Clin Microbiol. 2001 May.

Abstract

In this study, we compared serum hepatitis C virus (HCV) RNA concentrations with HCV RNA concentrations in whole blood collection tubes, including two different types of EDTA tubes and nucleic acid stabilization tubes (NASTs). We also investigated the impact of a processing delay on HCV RNA concentration in these tubes. In NASTs, the mean HCV RNA concentration was comparable to the mean serum HCV RNA concentration at "date zero." In EDTA tubes, mean baseline HCV RNA concentrations were higher. Storage at room temperature up to 96 h did not result in a decline of HCV RNA concentration in any of the whole blood collection tubes. In NASTs, HCV RNA concentrations remained stable during the whole study period, whereas a significant increase of HCV RNA was observed in both types of EDTA tubes at 96 h compared to date zero. We concluded that HCV RNA remains stable in NASTs at room temperature for at least 96 h, allowing greater flexibility in sample collection and transport.

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Figures

FIG. 1
FIG. 1
Effect of storage on different blood collection tubes. Symbols: ●, Vacuette EDTA; ▿, Vacuette NAST; ▪, Vacutainer EDTA.
See this image and copyright information in PMC

References

    1. Afonso A R M, Didier J, Plouvier E, Falissard B, Ferey M P, Bogard M, Dussaix E. Performance of an automated system for quantification of hepatitis C virus RNA. J Virol Methods. 2000;86:55–60. - PubMed
    1. Agnello V, Chung R T, Kaplan L M. A role for hepatitis C virus infection in type II cryoglobulinemia. N Engl J Med. 1992;327:1490–1495. - PubMed
    1. Albadalejo J, Alonso R, Antinozzi R, Bogard M, Bourgault A M, Colucci G, Fenner T, Petersen H, Sala E, Vincelette J, Young C. Multicenter evaluation of the COBAS AMPLICOR HCV assay, an integrated PCR system for rapid detection of hepatitis C virus RNA in the diagnostic laboratory. J Clin Microbiol. 1998;36:862–865. - PMC - PubMed
    1. Bouffard P, Hayashi P H, Acevedo R, Levy N, Zeldis J B. Hepatitis C virus is detected in a monocyte/macrophage subpopulation of peripheral blood mononuclear cells of infected patients. J Infect Dis. 1992;166:1276–1280. - PubMed
    1. Choo Q L, Weiner A J, Overby L R, Kuo G, Houghton M. Hepatitis C virus: the major causative agent of viral non-A, non-B hepatitis. Br Med Bull. 1990;46:423–441. - PubMed

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