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. 2001 May;39(5):1808-12.
doi: 10.1128/JCM.39.5.1808-1812.2001.

Sensitivity and specificity of human immunodeficiency virus rapid serologic assays and testing algorithms in an antenatal clinic in Abidjan, Ivory Coast

S Koblavi-Dème  1 , C Maurice, D Yavo, T S Sibailly, K N'guessan, Y Kamelan-Tano, S Z Wiktor, T H Roels, T Chorba, J N Nkengasong
Affiliations

Sensitivity and specificity of human immunodeficiency virus rapid serologic assays and testing algorithms in an antenatal clinic in Abidjan, Ivory Coast

S Koblavi-Dème et al. J Clin Microbiol. 2001 May.

Abstract

To evaluate serologic testing algorithms for human immunodeficiency virus (HIV) based on a combination of rapid assays among persons with HIV-1 (non-B subtypes) infection, HIV-2 infection, and HIV-1-HIV-2 dual infections in Abidjan, Ivory Coast, a total of 1,216 sera with known HIV serologic status were used to evaluate the sensitivity and specificity of four rapid assays: Determine HIV-1/2, Capillus HIV-1/HIV-2, HIV-SPOT, and Genie II HIV-1/HIV-2. Two serum panels obtained from patients recently infected with HIV-1 subtypes B and non-B were also included. Based on sensitivity and specificity, three of the four rapid assays were evaluated prospectively in parallel (serum samples tested by two simultaneous rapid assays) and serial (serum samples tested by two consecutive rapid assays) testing algorithms. All assays were 100% sensitive, and specificities ranged from 99.4 to 100%. In the prospective evaluation, both the parallel and serial algorithms were 100% sensitive and specific. Our results suggest that rapid assays have high sensitivity and specificity and, when used in parallel or serial testing algorithms, yield results similar to those of enzyme-linked immunosorbent assay-based testing strategies. HIV serodiagnosis based on rapid assays may be a valuable alternative in implementing HIV prevention and surveillance programs in areas where sophisticated laboratories are difficult to establish.

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Figures

FIG. 1
FIG. 1
(A) Results of the parallel serology algorithm using rapid assays. A true-positive serum was defined as concordantly positive by both rapid EIAs. A true-negative serum was defined as concordantly negative by both rapid EIAs. (B) Results of the serial serology algorithm using rapid assays. All serum specimens that were positive by Determine were further tested by Genie II and, when positive, were considered to be true positives. Specimens that were negative by Determine were reported as true negatives.
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