[Co-integration of BLG-LAtPA and WAP improved the expression of LAtPA in transgenic mouse milk]
[Article in
Chinese]
Affiliations
- PMID: 11330190
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[Co-integration of BLG-LAtPA and WAP improved the expression of LAtPA in transgenic mouse milk]
[Article in
Chinese]
Sheng Wu Gong Cheng Xue Bao.
2001 Jan.
Abstract
In order to improve the expression of longer acting tissue plasminogen activator in the mammary epithelium of transgenic mice, the fragment of BLG-LAtPA hydrid gene was microinjected into mouse embryos with mice whey acid protein gene. Three mouse were tested as being Co-integration of BLG-LAtPA and WAP transgene by PCR and Southern blot. Milk obtained from lactating females contains biologically active tPA, and the concentration of tPA was calculated to be about 10 micrograms/mL.
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