Characterization of the ToxB gene from Pyrenophora tritici-repentis
- PMID: 11332732
- DOI: 10.1094/MPMI.2001.14.5.675
Characterization of the ToxB gene from Pyrenophora tritici-repentis
Abstract
The ToxB gene was cloned and characterized from a race 5 isolate of Pyrenophora tritici-repentis from North Dakota. ToxB contains a 261-bp open reading frame that encodes a 23 amino acid putative signal peptide and a 64 amino acid host-selective toxin, Ptr ToxB. Analysis of Ptr ToxB from heterologous expression in Pichia pastoris confirms that ToxB encodes a host-selective toxin.