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. 2000;107(1-2):39-44.

Quantitative determination of urinary 8-hydroxydeoxyguanosine (8-OH-dg) by using ELISA

S Saito  1 , H Yamauchi, Y Hasui, J Kurashige, H Ochi, K Yoshida
Affiliations
  • PMID: 11334369

Quantitative determination of urinary 8-hydroxydeoxyguanosine (8-OH-dg) by using ELISA

S Saito et al. Res Commun Mol Pathol Pharmacol. 2000.

Abstract

In order to establish a quantitative assay for 8-OHdG concentrations in urine, we examined the precision of a test for the recovery of 8-OHdG in urine by using an ELISA method. The coefficient of variation (CV) for assay with incubation in water or air ranged between 7.0% and 8.4% and between 19.2% and 30.6%, respectively. The data by using incubation in water gave higher accuracy than those in air. The recovery rates of 8-OHdG in urine sample ranged from 95 - 114%. Our results indicated that the excellent sensitivity of this ELISA kit by using incubation in water makes its use possible for the determination in urine with a good reproducibility and recovery of 8-OHdG-spiked samples.

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