Comparative Study
doi: 10.1128/jcm.9.5.645-647.1979.
Rickettsial hemolysis: rapid method for enumeration of metabolically active typhus rickettsiae
- PMID: 113426
- PMCID: PMC275367
- DOI: 10.1128/jcm.9.5.645-647.1979
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Comparative Study
Rickettsial hemolysis: rapid method for enumeration of metabolically active typhus rickettsiae
J Clin Microbiol.
1979 May.
Abstract
A new assay is described for enumerating biologically active typhus rickettsiae (Madrid E strain), based on adsorption of rickettsiae to erythrocytes in the presence of NaF (which allows adsorption but not lysis) and lysis in the presence of anti-Rickettsia prowazeki immune serum (which allows only a single round of lysis). The number of lysed erythrocytes is then used to estimate the number of active rickettsiae.
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