doi: 10.1172/JCI107601.
Thyroid hormone action. Demonstration of similar receptors in isolated nuclei of rat liver and cultured GH1 cells
Affiliations
- PMID: 11344581
- PMCID: PMC301510
- DOI: 10.1172/JCI107601
Item in Clipboard
Thyroid hormone action. Demonstration of similar receptors in isolated nuclei of rat liver and cultured GH1 cells
J Clin Invest.
1974 Feb.
Abstract
High-affinity, limited-capacity nuclear binding activities, putative receptors for triiodothyronine, were detected after incubation of hormone with intact rat pituitary GH1 cells in culture, isolated GH1 cell nuclei, or rat liver nuclei. The total number of triiodothyronine binding sites per nucleus was similar in each case (approximately 8,000). The estimated equilibrium dissociation constants were virtually identical in isolated GH1 cell nuclei and rat liver nuclei, and both values were similar to that determined in intact GH2 cells. These results suggest that mechanisms of thyroid hormone action defined in cell culture could apply to thyroid hormone regulatory effects in vivo.
Similar articles
-
Thyroid hormone action: in vitro characterization of solubilized nuclear receptors from rat liver and cultured GH1 cells.J Clin Invest. 1974 Oct;54(4):853-65. doi: 10.1172/JCI107825. J Clin Invest. 1974. PMID: 4372251 Free PMC article.
-
Relationship of receptor affinity to the modulation of thyroid hormone nuclear receptor levels and growth hormone synthesis by L-triiodothyronine and iodothyronine analogues in cultured GH1 cells.J Clin Invest. 1979 Jun;63(6):1229-40. doi: 10.1172/JCI109418. J Clin Invest. 1979. PMID: 221536 Free PMC article.
-
Thyroid hormone receptors. Binding characteristics and lack of hormonal dependency for nuclear localization.J Biol Chem. 1975 Jun 10;250(11):4113-9. J Biol Chem. 1975. PMID: 236310
-
In vitro 3,5,3'-triiodothyronine binding to rat cerebrocortical neuronal and glial nuclei suggests the presence of binding sites unavailable in vivo.Endocrinology. 1985 May;116(5):2019-28. doi: 10.1210/endo-116-5-2019. Endocrinology. 1985. PMID: 2985367
-
L-triiodothyronine (T3) regulates cellular growth rate, growth hormone production, and levels of nuclear T3 receptors via distinct dose-response ranges in cultured GC cells.Endocrinology. 1990 May;126(5):2321-6. doi: 10.1210/endo-126-5-2321. Endocrinology. 1990. PMID: 2328689
Cited by
-
In vitro binding of L-triiodothyronine to receptors in rat liver nuclei. Kinectics of binding, extraction properties, and lack of requirement for cytosol proteins.J Clin Invest. 1975 Jan;55(1):50-60. doi: 10.1172/JCI107917. J Clin Invest. 1975. PMID: 162784 Free PMC article.
-
Nuclear receptors for thyroid hormone: evidence for nonrandom distribution within chromatin.Proc Natl Acad Sci U S A. 1975 May;72(5):1787-91. doi: 10.1073/pnas.72.5.1787. Proc Natl Acad Sci U S A. 1975. PMID: 168576 Free PMC article.
-
Thyroid hormone stimulates de novo growth hormone synthesis in cultured GH1 cells: evidence for the accumulation of a rate limiting RNA species in the induction process.Proc Natl Acad Sci U S A. 1976 Oct;73(10):3369-73. doi: 10.1073/pnas.73.10.3369. Proc Natl Acad Sci U S A. 1976. PMID: 185609 Free PMC article.
-
A new unextracted-sample radioimmunoassay method for hepatic endogenous nuclear L-tri-iodothyronine content. Validity of its use in determining nuclear receptor binding characteristics.Biochem J. 1982 Dec 15;208(3):641-9. doi: 10.1042/bj2080641. Biochem J. 1982. PMID: 6299277 Free PMC article.
-
Dose-dependent depletion of nuclear receptors by L-triiodothyronine: evidence for a role in induction of growth hormone synthesis in cultured GH1 cells.Proc Natl Acad Sci U S A. 1976 Nov;73(11):3877-81. doi: 10.1073/pnas.73.11.3877. Proc Natl Acad Sci U S A. 1976. PMID: 186776 Free PMC article.
References
Publication types
MeSH terms
Substances
Grants and funding
LinkOut - more resources
Full Text Sources
