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. 2001 Jun;69(6):4120-4.
doi: 10.1128/IAI.69.6.4120-4124.2001.

Vaccination with Bordetella pertussis-pulsed autologous or heterologous dendritic cells induces a mucosal antibody response in vivo and protects against infection

A George-Chandy  1 , N Mielcarek, I Nordström, J Holmgren, K Eriksson
Affiliations

Vaccination with Bordetella pertussis-pulsed autologous or heterologous dendritic cells induces a mucosal antibody response in vivo and protects against infection

A George-Chandy et al. Infect Immun. 2001 Jun.

Abstract

This study demonstrates for the first time that vaccination with either autologous or heterologous dendritic cells (DC) pulsed with specific antigen induces protective immune responses against noninvasive bacteria, namely Bordetella pertussis. The DC-mediated protection is associated with strong B. pertussis-specific immunoglobulin G (IgG) and IgA responses in the lung.

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Figures

FIG. 1
FIG. 1
B. pertussis-specific IgG titers in serum before (filled bars) and after (empty bars) challenge with live B. pertussis. Data are expressed as means + standard deviations. Triple asterisk, P < 0.001; double asterisk, P < 0.01, nontreated versus treated mice. Heterol., heterologous.
FIG. 2
FIG. 2
Overlay of fluorescence and light-microscopic image of DC pulsed with FITC-labeled B. pertussis and then counterstained with ethidium bromide. Ingested bacteria remain green, whereas extracellular bacteria become orange.
FIG. 3
FIG. 3
IFN-γ production by B. pertussis-specific T cells cocultured with B. pertussis-pulsed DC (black bars) or with unpulsed DC (white bars).
See this image and copyright information in PMC

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