doi: 10.1002/1522-2683()22:6<1021::AID-ELPS1021>3.0.CO;2-B.
Detection of Saccharomyces cerevisiae carboxylesterase activity after native and sodium dodecyl sulfate electrophoresis by using fluorescein diacetate as substrate
Affiliations
- PMID: 11358123
- DOI: 10.1002/1522-2683()22:6<1021::AID-ELPS1021>3.0.CO;2-B
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Detection of Saccharomyces cerevisiae carboxylesterase activity after native and sodium dodecyl sulfate electrophoresis by using fluorescein diacetate as substrate
Electrophoresis.
2001 Apr.
Abstract
A simple method for the visualisation of wine yeast esterase (carboxylesterase EC 3.1.1.1) activity on electrophoretic gels was developed, using the fluorescent substrate fluorescein diacetate. The zymogram system allows a sensitive detection of esterase bands in only 5 min of incubation of both native and sodium dodecyl sulfate gels.
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