Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2001;9(1):33-9.
doi: 10.1155/S1064744901000060.

Antimicrobial protein produced by vaginal Lactobacillus acidophilus that inhibits Gardnerella vaginalis

A A Aroutcheva  1 , J A Simoes, S Faro
Affiliations

Antimicrobial protein produced by vaginal Lactobacillus acidophilus that inhibits Gardnerella vaginalis

A A Aroutcheva et al. Infect Dis Obstet Gynecol. 2001.

Abstract

Objective: To isolate bacteriocin from a vaginal strain of Lactobacillus acidophilus.

Methods: L. acidophilus 160 was grown on two media. The first was MRS broth for 18 hours; the cells were harvested, washed, and placed into a chemically defined medium. The second medium resembled vaginal fluid minus protein. Bacteriocin was precipitated from both media using ammonium sulfate. The growth-inhibiting activity of bacteriocin was determined by a bioassay using nine different isolates of Gardnerella vaginalis.

Results: MRS broth is not a suitable medium for extracting bacteriocin, because it binds with Tween 80. Bacteriocin was isolated, without contaminating constituents, from chemically defined medium and identified as a single band by electrophoresis. Bacteriocin has a molecular weight of 3.8 kDa. All nine isolates of Gardnerella were inhibited by the bacteriocin isolated from L. acidophilus 160.

Conclusions: Bacteriocin produced by L. acidophilus 160 was isolated from the chemically defined medium (starvation medium) in a partially pure form. L. acidophilus 160 bacteriocin inhibited growth of all nine isolates of Gardnerella vaginalis.

PubMed Disclaimer

References

    1. Acta Pathol Microbiol Immunol Scand B. 1986 Dec;94(6):399-403 - PubMed
    1. Appl Environ Microbiol. 1983 Jun;45(6):1808-15 - PubMed
    1. Antimicrob Agents Chemother. 1987 Aug;31(8):1231-3 - PubMed
    1. Can J Microbiol. 1988 Aug;34(8):974-8 - PubMed
    1. Appl Environ Microbiol. 1991 Jan;57(1):114-21 - PubMed

MeSH terms

LinkOut - more resources