Dendritic responses of frog motoneurons produced by antidromic activation

Abstract

Responses to ventral root stimulation were studied in spinal cords in situ in unanesthetized frogs. Extracellular as well as intracellular recordings from motoneurons indicated that considerable depolarization of the dendrites occurred in the response to ventral root volley. Active components of this dendritic depolarization could also be observed. Extracellularly, negative field potentials were recorded both in the vicinity of motoneuron cell bodies and in areas occupied mostly by motoneuron dendrites. The refractory period of the negativity recorded at the vicinity of dendrites was longer than in the vicinity of somata. Changes in antidromic excitability were studied by the double volley technique. Augmentation of the field potential to a test volley was observed during the period of dendritic depolarization, followed by a longer-lasting period of depression of the test response. It is concluded that an action potential in frog motoneurons induces depolarization of the dendrites. The depolarized dendrites can generate local action potentials and can produce negative field potentials remotely from the somatic pool. The response of dendrites to stimulation of the ventral root has particular importance in the recurrent facilitation of the frog motoneurons.